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Studies of the Atomic and Crystalline Characteristics of Ceramic Oxide Nano Powders after Bio Field Treatment


Industrial Engineering & Management
Received April 23, 2015; Accepted June 15, 2015; Published June 17, 2015


Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the Atomic and Crystalline Characteristics of Ceramic Oxide Nano Powders after Bio field Treatment. Ind Eng Manage 4: 161. doi:10.4172/2169-0316.1000161


Studies of the Atomic and Crystalline Characteristics of Ceramic Oxide Nano Powders after Bio Field Treatment

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Transition metal oxides (TMOs) have been known for their extraordinary electrical and magnetic properties. In the present study, some transition metal oxides (Zinc oxide, iron oxide and copper oxide) which are widely used in the fabrication of electronic devices were selected and subjected to biofield treatment. The atomic and crystal structures of TMOs were carefully studied by Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD) studies. XRD analysis reveals that biofield treatment significantly changed the lattice strain in unit cells, crystallite sizes and densities in ceramics oxide powders. The computed molecular weight of the treated samples exhibited significant variation. FT-IR spectra indicated that biofield treatment has altered the metal-oxygen bond strength. Since biofield treatment significantly altered the crystallite size, lattice strain and bond strength, we postulate that electrical and magnetic properties in TMOs (transition metal oxides) can be modulated by biofield treatment.

Keywords: Biofield treatment; Iron oxide; Copper Oxide; Zinc Oxide; X-ray diffraction; FT-IR


Transition metal oxides (TMOs) exhibit fascinating properties such as piezoelectricity, ferroelectricity, nonlinear optical behaviour, wide band gap and high-TC superconductivity, which allows these metal oxides to use in electronic and optical device industries [1]. Zinc oxide (ZnO) is a common example of TMO which has been used in fabrication of light emitting diodes (LEDs), Varistors (a ceramic resistor which used to protect electronic circuits from high voltages) due to its wide band gap (3.37eV) and large excitation binding energy [2-4]. On the other hand iron oxide (Fe3O4) is mainly utilized in fabrication of biosensors due to its low inherent toxicity, biocompatibility and strong paramagnetic behaviour [5]. Copper oxide has been used as a p-type semiconductor for the fabrication of electrical, photovoltaic devices, field emission devices due to its narrow band gap of 1.5 eV and high optical absorption coefficient [6]. Moreover, in TMOs the magnetic, electrical and optical properties are directly influenced by lattice strain and crystallite size [7,8].

ZnO using in Varistors requires low leakage current during continuous operating voltage and uniform current distribution for better performance, which is possible by controlling its crystallite size [7]. Energy band gaps in ZnO and CuO can be controlled by increasing or decreasing the lattice parameter (Energy band gap is inversely proportional to lattice parameter) [8]. Researchers have optimized band gap in ceramics by controlling the composition of certain alloys and lattice constant of unit cell. It is known that tailoring the energy band gap in ceramic oxides is crucial to create barrier layers and quantum wells with materials of matching properties such as electron affinity and lattice constant [9,10]. Recently, TMOs nanoparticles are synthesized by electrophoretic deposition, laser ablation, anodization hydrothermal methods, electrochemical depositions sol–gel method, microwave-assisted combustion chemical vapour deposition, thermal decomposition, combustion method, ultrasound method and coprecipitation as per various literatures, wherein correlation of band gap with crystal structure and morphology has been studied [11-21].

In the prior published studies, Biofield treatment has been used to cause the changes in the atomic and structural properties such as lattice constant, bond strength, and molecular weight. Recently it was reported that a robotic quad copter in space can be controlled through the power of thoughts [22]. Trivedi’s biofield, referred herein as biofield treatment, is known to transform the atomic, molecular, structural properties of various metals and ceramics in the field of material science [23-30], such as it has enlarged the particle size by 432% in zinc powder [23] and enhanced the crystallite size by 66% in vanadium pentoxide [27]. Additionally, the biofield treatment also achieved excellent results in various other fields such as microbiology [31-33], biotechnology [34,35] and agriculture [36-38] etc., which are reported elsewhere.

In the present investigation effect of Trivedi’s biofield treatment on three TMOs nanopowders, (Zinc oxide, Iron oxide and copper oxide) are studied at the atomic and structural level.

Experimental Procedure

Iron oxide, copper oxide and zinc oxide nanopowders used in the present investigation were procured from Sigma Aldrich which had purity of around 99% which were distributed into three sets, out of which one set was considered as control. And other two sets of each oxide were exposed to Mr. Trivedi’s biofield, referred hereinafter as treated T1 and treated T2.

All samples were characterized by X-ray diffraction (XRD) Phillips, Holland PW 1710 with copper anode, nickel filter and radiation of wavelength of 1.54056 Å. XRD evaluated result was obtained in the form of intensity vs. 2θ with a table containing d values, 2θ°, peak width 2θ°, relative Intensity %, peak intensity counts, etc. For data analysis, Standard JCPDS database and Miller indices h, k, l were used to get the value of “d” and Powder X software was used to calculate lattice parameter and unit cell volume. Crystallite size (size of a grain) was computed as: Crystallite size=k λ/b Cos θ

Here λ is the wavelength of x-radiation k is the equipment constant with a value 0.94.

Percent change in unit cell volume was calculated as 100 × (ΔV/ Vc) where ΔV=[(Vt-Vc)/Vc] where Vt and Vc are the unit cell volume of treated and control samples respectively. Similarly percent change in lattice parameter, molecular weight, density was computed. The atomic weight of an atom is calculated as the sum of weights of all protons, neutron and electron present in an atom and molecular weight was calculated as the sum of atomic weights of all atoms present in a molecule. Density was calculated as molecular weight multiplied by number of molecules in a unit cell divided by unit cell volume.

The infrared spectra of metal oxide powders were carried out using with Perkin Elmer, USA Fourier Transform Infrared (FT-IR) Spectrometer, which recorded data in the range of 500-4000/cm.

Results and Discussion
X-ray diffraction (XRD)

Figure 1 shows the comparative results of lattice parameter ‘a’ of various control and treated ceramic nanopowders with the values obtained from standard JCPDS (Joint Committee on Powder Diffraction Standards) data. It was found that the value of lattice parameter “a” of control powder was closely matched with the standard JCPDS values. However in case of treated nanopowder samples, the biofield treatment has induced changes in lattice parameter to second decimal place (Table 1).

The percent variation in lattice parameter of treated samples is shown in Figure 2. It was observed that the lattice parameter was significantly decreased by 0.6%, 0.39% and 0.04% in Fe3O4, CuO, and ZnO powders, respectively. The volume of the unit cell was calculated using the parameter ‘a’ that showed similar variation as given in Table 1.The decrease in lattice parameters of all the treated powders indicated that a compressive stress has been applied through biofield treatment.The percent change in linear strain (100 × (Δa/ac) would be significant, if it’s value is above 0.2% ,which is considered to be above the elastic limit. Once applied stress over unit cell is removed, the elastic strain is expected to release, which did not happen in present investigation. Rather the strain was permanent, which revealed that it is plastic by nature, but the ceramics are elastic materials [39,40]. The absence of dislocations in covalent ceramics and the narrow dislocation width in ionic ceramics make this exhibit elastic behavior.

Figure 1 Comparison of lattice parameter ‘a’ of treated ceramic nanopowders with standard JCPDS data and control.

Figure 1: Comparison of lattice parameter ‘a’ of treated ceramic nanopowders with standard JCPDS data and control.

Percent change in lattice parameter ‘a’ between control and treated ceramic nanopowders

Figure 2: Percent change in lattice parameter ‘a’ between control and treated ceramic nanopowders.

Percent change crystallite size between control and treated powders

Figure 3: Percent change crystallite size between control and treated powders.

The percent changes in crystallite size are presented in Figure 3. In treated samples, it was found that the crystallite size was significantly increased up to 275.26%, 98.92% and 77.7% in Fe3O4, CuO, and ZnO powders, respectively. The higher crystallite size in treated samples suggests heavy plastic deformation along with large expansion and growth.

The ceramics are elastic material, and their dislocation concentration is minimal. Furthermore high energy is required to move the dislocations in ceramic crystals than crack propagation; hence ceramics fails by brittle fracture rather than plastic deformation. The results showed that ceramic crystallite size was increased, may be due to re-crystallization and crystallite growth which might have been possible in the presence of large amount of energy (localized heat to at least above one third of melting point) in the crystals, provided by the biofield treatment. Another reason may be the rotation of crystallites at their boundaries aided by viscous flow which has been known to occur in nano crystalline solids. Whatever be the mechanism the decrease in lattice parameters and increase in crystallite size are opposing effects, which is an unusual phenomena. So it is necessary to study the characteristics at the atomic level.

The lattice parameters are considered as the equilibrium inter atomic distance where the attractive forces of nuclear charges of metal ions are balanced by the negative charges of metal ions. The effective nuclear charge on the metal ion was reduced with increasing distance from the nucleus. When compressive force has been applied over atoms then electron clouds are likely to be pushed toward the nucleus,that lead to further reduction in the size of the atoms and cause an increase in the nuclear charge per unit volume, whereas reverse may happen in case of tensile stress. The calculated values of the atomic parameters and their percentage variation between control and treated samples are given in Tables 1 and 2 and Figures 4-6.













Crystal Structure







Lattice parameter ‘a’ x 10-8 cm

% change in ‘a’

JCPDS Standard








Treated T1







Treated T2







Volume of unit cell  x 10-24 cm3

% change in volume of unit cell





Treated T1







Treated T2







Crystallite size ‘g’ x 10-9 m

% change in ‘g’





Treated T1







Treated T2







Table 1 Analysis of x-ray diffraction results of oxide nano powders crystal parameters













Molecular weight (g/mol)

% change in molecular weight





Treated T1







Treated T2







Density g/cc

% change in density





Treated T1







Treated T2







Table 2. Analysis of x-ray diffraction results of oxide nano powders- molecular weight and density

It was observed that unit cell volume was decreased in iron oxide and zinc oxide due to decrease in lattice parameter “a”. Contrarily the unit cell volume was increased in copper oxide due to increase in lattice parameter “c”. Furthermore, the change in unit cell volume affects the size of atom that changes the effective nuclear charge per unit volume of the metal ions accordingly. The observed decrease in molecular weight and density was mainly attributed to the change in proton to neutron ratio inside the nucleus. This could be due to weak reversible nuclear level reaction, which occurred inside the nucleus. Thus, we presume that biofield has transferred the energy in the form of neutrinos that possibly caused this nuclear level reaction to alter their ratio. Furthermore, it is already reported that the crystallite size and lattice strain in ceramic nano oxides directly affects the energy band gap and piezoelectric properties. The higher crystallite size indicates lower energy band gap, which make these oxides more useful in semiconductors [6,9].

FT-IR spectroscopy 

FT-IR spectrum of zinc oxide (control) and treated sample is illustrated in Figure 7. FT-IR of the zinc oxide (control) exhibited absorption peaks at 547/cm, 496/cm and 440/cm, which were attributed to the characteristic Zn-O stretching vibrations peaks [41]. Whereas treated sample of zinc oxide showed an emergence of a broad peak at 3410/cm which was responsible for O-H stretching vibrations. Here, we presume that biofield treatment is responsible for increasing the surface to volume ratio of ZnO leading to enhanced moisture absorption [42,43] by the treated sample. Other important peaks were observed at 425/cm, 547/cm could be ascribed to Zn-O stretching vibration, and absorption peaks at 878/cm and 1275/cm corresponds to H-O-H and O-H bending vibration, respectively [42]. The absorption band of control and treated sample of ZnO was observed at 440/cm and 425/ cm, respectively. The treated ZnO sample showed lower wavenumber (425/cm), that may be due to unit cell distortion or change in Zn—O bond length [44].

Percent change in unit cell volume between control and treated ceramic nanopowders

Figure 4: Percent change in unit cell volume between control and treated ceramic nanopowders.

Percent change in molecular weight (Change in weight of total number of protons and neutrons) of treated ceramic nanopowders

Figure 5: Percent change in molecular weight (Change in weight of total number of protons and neutrons) of treated ceramic nanopowders.

Percentage change in density of control and treated ceramic nanopowders

Figure 6: Percentage change in density of control and treated ceramic nanopowders.

FT-IR spectrum of control and treated samples of zinc oxide

Figure 7: FT-IR spectrum of control and treated samples of zinc oxide.

FT-IR spectrum of control and treated samples of copper oxide

Figure 8: FT-IR spectrum of control and treated samples of copper oxide.

FT-IR spectrum of control and treated samples of iron oxide

Figure 9: FT-IR spectrum of control and treated samples of iron oxide.

FT-IR spectrum of copper oxide is shown in Figure 8. The FT-IR of copper oxide (control) has showed only one peak at 619/cm, which was mainly due to Cu (II)-O stretching vibrations peak [45]. Contrarily in treated copper oxide sample, this absorption peak was observed at higher wavenumber, 629/cm that may have been due to a reduction in particle size or strengthening of Cu—O bond through biofield treatment [46].

The FT-IR spectrum of Iron oxide is illustrated in Figure 9. The Iron oxide (control) sample showed intense absorptions peaks at 619/ cm and 568/cm respectively which could be due to Fe-O stretching vibrations in Fe3O4 [47]. The treated iron oxide sample exhibited peaks at 644.4/cm and 568/cm respectively which were attributed to the Fe—O vibration peaks [47,48]. This may be due to contributions from the stretching vibration bands related to metal in the tetrahedral and octahedral sites of the oxide structure. However in treated Fe3O4, the absorption band responsible for Fe-O bond shifted towards higher wavenumber of 644/cm, which may be possible due to breaking of many bonds on surface atoms. This may cause rearrangement of localized electrons on the particle surface leading to increase in surface bond force constant; followed by the shifting of absorption bands to higher wavenumber [49]. It is hypothesized that energy through biofield treatment has been transferred to atoms, which caused these changes in bond properties.

In present work, biofield treatment altered the atomic and structural properties, which may lead to the changes in corresponding optical and electrical properties in ceramic nanopowders.


Biofield treatment by Mr. Trivedi has significantly altered the atomic and crystalline characteristics of nano Iron oxide (Fe3O4), nano copper oxide (CuO) and nano zinc oxide (ZnO) as follows;

• As per the results, the decrease in volume of unit cell up to 1.79% and an increase in crystallite size by 275.26% are opposing effects and can be only explained based on current knowledge in terms of dislocation movements during recovery and re-crystallization. This is an unprecedented phenomenon observed, since ceramics are elastic solids.

• The treated powders exhibited increase as well decrease in volume of the unit cell and molecular weight. The changes in density are exactly reversed. This inverse relationship between density and molecular weight can only be explained by changes in the total number of neutrons and protons as well as their ratio. So we hypothesize that the results achieved could be due to changes in protons and neutrons in the nucleus caused by weak interactions.

• FT-IR spectra showed, significant shifting of M-O stretching vibration in all transition metal oxides (TMOs), which revealed that biofield treatment have altered the bond length and bond force constant.

• Based on the higher crystallite size and higher lattice strain achieved in treated ZnO, Fe3O4 and CuO powders ; we assume that these ceramics could be used for piezoelectric devices, ferrofluids, and semiconductor applications, respectively.


We sincerely thank to all the laboratory staff of various laboratories for their active support in characterizing the samples. We thank Dr. Cheng Dong of NLSC, Institute of Physics, and Chinese Academy of Sciences for permitting us to use PowderX software for analyzing XRD results.


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